We use multi-angle light scattering (MALS) detector exclusively connected on-line to size exclusion chromatography (SEC) and asymmetric flow flied-flow fractionation (AF4). MALS is used in the analysis of a wide range of compounds such as proteins, nanoparticles, viruses, and polymers. Our MALS detector uses 18-angles and is from Wyatt Technology.
Multi-angle light scattering (MALS) is a detection technique used to characterize macromolecules and particles based on their scattering light. The amount of scattering of light will depend on the molecular weight and size of the macromolecule or particle, among others.
Multi-angle light scattering (MALS) is applicable to compounds with molecular weight of ~ 1 000 g/mol and upwards to sizes of approximately 500 nm. It is typically used connected on-line to chromatography techniques (SEC and AF4) to measure the molar mass and size of the eluted compounds.
- Molar mass information without standards
- High sensitivity for high molar mass components and aggregates
- Excellent for troubleshooting SEC and AF4
- Specialized and expensive equipment
- Sensitivity to particulate impurities
- Require some experience to operate and for data evaluation
- Poor sensitivity for low molar mass components